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born 1955 Ivory Coast
Pascale Guiton is a microbiologist and assistant professor at Santa Clara University, where she focuses on both teaching and research. She specializes in parasitology, particularly the study of Toxoplasma gondii.
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I am not very focused and have lots of questions, but I now have teams of undergraduate students working on each of these questions.
I started thinking ‘I'm going to have to pay for all these things’.
I thought, ‘oh, that's cool; can I know more about these things.
I would say that the transition was not too bad for me at first.
My primary objective is to fully engage my students so that they can acquire these values and skills that transcend degree requirements.
I can share my passion for microbiology, introduce students to the rigor and the thrill of scientific research, discovery, and innovation.
We just started this project, which I am very excited about.
I need to publish’ but more as an extension of my teaching.
She really believed in me, which I am really grateful for.
If we need to make some knockout lines, I have to teach them about bacterial growth, aseptic technique, PCR, gel electrophoresis, CRISPR-Cas9.
Another feature of Toxoplasma is the presence of specialized secretory organelles (micronemes, rhoptries and dense granules) found in the apical complex.
Toxoplasma can infect orally using two forms, the product of sexual reproduction (the oocyst) that eventually sporulate to produce infectious sporozoites.
There are many aspects of T. gondii biology we know very little about.
I had this natural curiosity and wanted to know more.
I had been in labs that were pretty established.
As I became more established, I had more people in my lab to look after, more students to teach and more grants to write.
It was quite daunting; I was also sitting on departmental and college committees, advising student clubs, writing grants, mentoring students in the lab.
I did have a startup package, but as East Bay is a teaching institution, it wasn't as high as you would expect compared to research-intensive institutions.
When you ingest Toxoplasma, it has to get through the microbiota, cross the mucous layer, get to the epithelium, cross the epithelium, find its way into the blood.
My approach to effective teaching and learning is akin to manoeuvring a railway handcar whereby both learner and teacher work as partners in an environment conducive to learning.
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